Composite

Part:BBa_K360143:Design

Designed by: Jorge Eduardo Buendía Buendía   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-22)


J23101 promoter + Strong RBS + GFP E0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704


Design Notes

See design considerations of the strong RBS and spacer.


Source

Promoter BBa_J23101 and GFP BBa_E0040 were taken from the registry. Strong RBS BBa_K360031 was taken from lacZ gene of Escherichia coli

References